Abstract
Human biomonitoring studies to determine total titanium (total-Ti) and titanium dioxide (TiO2) particles require very sensitive inductively coupled plasma high resolution mass spectrometry (ICP-HRMS) methods. The result of such studies can only be reliably when used in a risk assessment if the analytical methods are fully validated, as reported here for human liver and spleen. For total-Ti an acidic HNO3/HF digestion was applied, followed by ICP-HRMS analysis of the acidic digests. For TiO2 particles a two-step digestion procedure was developed. In the first step the formaldehyde-fixed tissue was depolymerized and in the second step an enzymatic digestion was performed. Sample digests were analyzed with ICP-HRMS operated in single particle mode to detect TiO2 particles. Both methods were validated according to NEN 7777 using a scheme of duplicate analysis over an eight day period. Detection limits for total-Ti and TiO2 particles were 0.01 and 0.005 mg Ti/kg with a particle size quantification limit of 85 nm for TiO2 particles. Repeatability and reproducibility were 24% and 31% for the total titanium concentration, 22% and 39% for the particle titanium concentration, and 29% and 24% for the titanium dioxide particle size. In total 30 human tissue samples were analyzed with the developed and validated method. The results show a total-Ti content in the range of 0.02-0.09 mg Ti/kg in liver and 0.02-0.4 mg Ti/kg in spleen. The concentrations of particle TiO2 ranged from 0.01-0.08 mg Ti/kg in liver and from 0.01-0.1 mg Ti/kg in spleen. On the average, particle TiO2 explained up to 67% of the total-Ti concentrations. The detected TiO2 particles had a particle size range of 85 to 720 nm.
| Original language | English |
|---|---|
| Journal | Current Trends in Analytical and Bioanalytical Chemistry |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2018 |
Access to Document
10.36959/525/442Licence: CC BY
https://edepot.wur.nl/519370Licence: CC BY
Fingerprint
Dive into the research topics of 'Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS'. Together they form a unique fingerprint.
Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver
Peters, R., Undas, A., Memelink, J., Van Bemmel, G., Munniks, S., Bouwmeester, H., Nobels, P., Schuurmans, W., & Van Der Lee, M. (2018). Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS. Current Trends in Analytical and Bioanalytical Chemistry, 2(1). https://doi.org/10.36959/525/442
Peters, Ruud ; Undas, Anna ; Memelink, Joost et al. / Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS. In: Current Trends in Analytical and Bioanalytical Chemistry. 2018 ; Vol. 2, No. 1.
@article{2de6a67421b849758652a1ba2d2f4b30,
title = "Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS",
abstract = "Human biomonitoring studies to determine total titanium (total-Ti) and titanium dioxide (TiO2) particles require very sensitive inductively coupled plasma high resolution mass spectrometry (ICP-HRMS) methods. The result of such studies can only be reliably when used in a risk assessment if the analytical methods are fully validated, as reported here for human liver and spleen. For total-Ti an acidic HNO3/HF digestion was applied, followed by ICP-HRMS analysis of the acidic digests. For TiO2 particles a two-step digestion procedure was developed. In the first step the formaldehyde-fixed tissue was depolymerized and in the second step an enzymatic digestion was performed. Sample digests were analyzed with ICP-HRMS operated in single particle mode to detect TiO2 particles. Both methods were validated according to NEN 7777 using a scheme of duplicate analysis over an eight day period. Detection limits for total-Ti and TiO2 particles were 0.01 and 0.005 mg Ti/kg with a particle size quantification limit of 85 nm for TiO2 particles. Repeatability and reproducibility were 24% and 31% for the total titanium concentration, 22% and 39% for the particle titanium concentration, and 29% and 24% for the titanium dioxide particle size. In total 30 human tissue samples were analyzed with the developed and validated method. The results show a total-Ti content in the range of 0.02-0.09 mg Ti/kg in liver and 0.02-0.4 mg Ti/kg in spleen. The concentrations of particle TiO2 ranged from 0.01-0.08 mg Ti/kg in liver and from 0.01-0.1 mg Ti/kg in spleen. On the average, particle TiO2 explained up to 67% of the total-Ti concentrations. The detected TiO2 particles had a particle size range of 85 to 720 nm. ",
author = "Ruud Peters and Anna Undas and Joost Memelink and {Van Bemmel}, Greet and Sandra Munniks and Hans Bouwmeester and Peter Nobels and Wobbe Schuurmans and {Van Der Lee}, Martijn",
year = "2018",
doi = "10.36959/525/442",
language = "English",
volume = "2",
journal = "Current Trends in Analytical and Bioanalytical Chemistry",
issn = "2642-4266",
number = "1",
}
Peters, R, Undas, A, Memelink, J, Van Bemmel, G, Munniks, S, Bouwmeester, H, Nobels, P, Schuurmans, W & Van Der Lee, M 2018, 'Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS', Current Trends in Analytical and Bioanalytical Chemistry, vol. 2, no. 1. https://doi.org/10.36959/525/442
Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS. / Peters, Ruud; Undas, Anna; Memelink, Joost et al.
In: Current Trends in Analytical and Bioanalytical Chemistry, Vol. 2, No. 1, 2018.
Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS
AU - Peters, Ruud
AU - Undas, Anna
AU - Memelink, Joost
AU - Van Bemmel, Greet
AU - Munniks, Sandra
AU - Bouwmeester, Hans
AU - Nobels, Peter
AU - Schuurmans, Wobbe
AU - Van Der Lee, Martijn
PY - 2018
Y1 - 2018
N2 - Human biomonitoring studies to determine total titanium (total-Ti) and titanium dioxide (TiO2) particles require very sensitive inductively coupled plasma high resolution mass spectrometry (ICP-HRMS) methods. The result of such studies can only be reliably when used in a risk assessment if the analytical methods are fully validated, as reported here for human liver and spleen. For total-Ti an acidic HNO3/HF digestion was applied, followed by ICP-HRMS analysis of the acidic digests. For TiO2 particles a two-step digestion procedure was developed. In the first step the formaldehyde-fixed tissue was depolymerized and in the second step an enzymatic digestion was performed. Sample digests were analyzed with ICP-HRMS operated in single particle mode to detect TiO2 particles. Both methods were validated according to NEN 7777 using a scheme of duplicate analysis over an eight day period. Detection limits for total-Ti and TiO2 particles were 0.01 and 0.005 mg Ti/kg with a particle size quantification limit of 85 nm for TiO2 particles. Repeatability and reproducibility were 24% and 31% for the total titanium concentration, 22% and 39% for the particle titanium concentration, and 29% and 24% for the titanium dioxide particle size. In total 30 human tissue samples were analyzed with the developed and validated method. The results show a total-Ti content in the range of 0.02-0.09 mg Ti/kg in liver and 0.02-0.4 mg Ti/kg in spleen. The concentrations of particle TiO2 ranged from 0.01-0.08 mg Ti/kg in liver and from 0.01-0.1 mg Ti/kg in spleen. On the average, particle TiO2 explained up to 67% of the total-Ti concentrations. The detected TiO2 particles had a particle size range of 85 to 720 nm.
AB - Human biomonitoring studies to determine total titanium (total-Ti) and titanium dioxide (TiO2) particles require very sensitive inductively coupled plasma high resolution mass spectrometry (ICP-HRMS) methods. The result of such studies can only be reliably when used in a risk assessment if the analytical methods are fully validated, as reported here for human liver and spleen. For total-Ti an acidic HNO3/HF digestion was applied, followed by ICP-HRMS analysis of the acidic digests. For TiO2 particles a two-step digestion procedure was developed. In the first step the formaldehyde-fixed tissue was depolymerized and in the second step an enzymatic digestion was performed. Sample digests were analyzed with ICP-HRMS operated in single particle mode to detect TiO2 particles. Both methods were validated according to NEN 7777 using a scheme of duplicate analysis over an eight day period. Detection limits for total-Ti and TiO2 particles were 0.01 and 0.005 mg Ti/kg with a particle size quantification limit of 85 nm for TiO2 particles. Repeatability and reproducibility were 24% and 31% for the total titanium concentration, 22% and 39% for the particle titanium concentration, and 29% and 24% for the titanium dioxide particle size. In total 30 human tissue samples were analyzed with the developed and validated method. The results show a total-Ti content in the range of 0.02-0.09 mg Ti/kg in liver and 0.02-0.4 mg Ti/kg in spleen. The concentrations of particle TiO2 ranged from 0.01-0.08 mg Ti/kg in liver and from 0.01-0.1 mg Ti/kg in spleen. On the average, particle TiO2 explained up to 67% of the total-Ti concentrations. The detected TiO2 particles had a particle size range of 85 to 720 nm.
U2 - 10.36959/525/442
DO - 10.36959/525/442
M3 - Article
SN - 2642-4266
VL - 2
JO - Current Trends in Analytical and Bioanalytical Chemistry
JF - Current Trends in Analytical and Bioanalytical Chemistry
IS - 1
ER -
Peters R, Undas A, Memelink J, Van Bemmel G, Munniks S, Bouwmeester H et al. Development and Validation of a Method for the Detection of Titanium Dioxide Particles in Human Tissue with Single Particle ICP-MS. Current Trends in Analytical and Bioanalytical Chemistry. 2018;2(1). doi: 10.36959/525/442
